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Runs uploaded in manual method have their facts instantly handed to bcl2fastq without having more processing.
When making use of IEM, i5 sequences have to be entered into these software program during the ahead orientation in all situation and for all instrument sorts.
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csv file or the SampleSheet.csv file created by LRM within the Assessment start out. LRM will detect the instrument variety through the run data files and create a final sample sheet While using the i5 indexes in the proper orientation for that platform.
Our instrument functionality company helps minimize unplanned downtime and lessen instrument requalification
If operating in handbook mode and using bcl2fastq or BCL Transform, or uploading the sample sheet to BaseSpace to be a guide mode operate and attaching a sample sheet, enter the i5 sequence during the ahead route.
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This means that these computer software Focus on the belief that your i5 sequence is always from the forward orientation and will automatically create the reverse complement with the sequences supplied for the appropriate instrument type.
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Library prep, enrichment, and probe panel reagents need to be purchased separately. Suitable library prep kits: